2021 SGMS Meeting
The SGMS board has started planning the 2021 meeting and update the website as soon as possible. The meeting will be preceeded by the 3rd SGMS School on 17 November 2021.
Confirmed Plenary Speakers for 2021 (we are currently trying to reconfirm them... stay tuned)
- Joe Loo, UCLA
- Thorsten Benter, University of Wuppertal (abstract)
- Ralf Weber, University of Birmingham (abstract)
- Silke Grabherr, University of Lausanne (abstract)
Registration is now open. Please register using theRegistration Form
Send an e-mail to registration(at)sgms(dot)ch should you encounter problems with registration and/or abstract submission.
- Early abstract registration: July 15th (Poster/Oral acceptance will be notified by September 1st).
- Abstract registration: September 1st (Poster/Oral acceptance will be notified by September 21st).
- Standard registration: August 31st (Includes one night at the Dorint hotel, Thursday Apéro and SGMS dinner, Friday breakfast, coffee breaks).
- Late registration: November 15th
Short Oral Contributions: Early deadline for abstract submission for both talks and posters is July 15th. The extended deadline is September 1st. Abstracts submitted before July 1st will have priority.
Guidelines for the submission of abstracts: Submit your abstract at the time of registration. The abstract should not exceed 2500 characters.
Posters: Poster size should not exceed 146 H x 118 W cm (size of pin wall). There will be a defined poster session.
|Single Room Occupancy||300||350|
|Double Room Occupancy||270||320|
|Student (double room - indicate roommate)||100|
|Accompanying person (indicate roommate)||230|
A surcharge of CHF 50 will be enforced to all payments submitted after the meeting.
Joe Loo: tba
Department of Chemistry & Biochemistry and Department of Biological Chemistry
David Geffen School of Medicine at the University of California
Los Angeles (UCLA)
United States of America
Physical Chemistry & Theoretical Chemistry
Faculty for Mathematics and Natural Sciences
University of Wuppertal
Even today, a comprehensive and congruent model for the electrospray ionization (ESI) process explaining all experimental observations is not established. This is due to the fact that the “ESI process” does not only include the (liquid phase) ionization of a molecule but also the liquid-gas phase transfer - including fluid dynamics and interface- and electrochemistry. The formation and evolution of charged droplets, the release of ions from charged droplets, the transport of ions/droplets into the vacuum system of a mass spectrometer, ion activation, transformation (i.e., chemistry), and means of preparing a defined ion beam in the ion transfer stage all potentially impact on the observed mass spectrum.
A phenomenon coined in the literature as supercharging may yield new insights in the formation processes of multiply charged ions from ESI. Supercharging is “used” to generate highly charged ion species, which may then be subjected to selected fragmentation methods (e.g., ETD, ECD). There are two different approaches to achieve supercharging: The conventional way is adding supercharging agents (SCAs) to the sprayed analyte solution. In contrast, supercharging is also achieved by adding gas phase components (e.g. acetonitrile) into the ion source.
This presentation focuses on the systematic investigation of the impact of liquid and gas phase modifiers on the observed ion population and changes of the average charge state of the peptide Substance P (SP, sequence: RPKPQQFFGLM). SP is used as a model analyte since it is well characterized with regard to its structure and ionization behavior. In addition, proxies of individual motives of SP, e.g., 1,5-diaminopentane, ethylenediamine, n-butylamine, are also investigated with regard to chemically induced charge state changes.
Results from experimental as well as theoretical work [1,2] are presented and discussed.
 Thinius, M. et al.; “Charge Retention/Charge Depletion in ESI-MS – Experimental Evidence”; J. Am. Chem. Soc. Mass Spectrom. DOI: 10.1021/jasms.9b00044.
 Haack, A. et al.; “Charge Retention/Charge Depletion in ESI-MS – Theoretical Rationale”; J. Am. Chem. Soc. Mass Spectrom. DOI: 10.1021/jasms.9b00045.
Ralf Weber: Towards More Complete Annotation of Model Organism Metabolomes: Analytical and Computational Approaches
School of Biosciences & Phenome Centre Birmingham
University of Birmingham
Our knowledge of the metabolic composition of model organisms, or even human biofluids, remains remarkably limited. Identifying the collection of metabolites that make up these matrices remains one of the greatest roadblocks to deriving biological knowledge from non-targeted metabolomics datasets. Calls for community-driven characterisation of model organism metabolomes have recently been issued to address this fundamental knowledge gap. Here we present a novel analytical and computational workflow – the Deep Metabolome Annotation (DMA) workflow – for characterising (model organism) metabolomes using extensive separation and analytical techniques, as well as an extensive set of existing and novel computational tools to achieve large-scale metabolome annotation in an untargeted manner.
The DMA workflow takes as input a single, complex biological sample matrix, from which polar and apolar metabolites are independently extracted. Resulting extracts are each fractionated over two distinct solid-phase extraction cartridges. SPE fraction aliquots are, in turn, further fractionated using multiple, complementary liquid chromatography methods, with concurrent fraction collection and high-resolution tandem mass spectrometry (MS) analysis. Finally, resulting liquid chromatography fractions are infused into OrbitrapTM platforms for extensive high-resolution tandem and multiple-stage MS (MSn) analyses (i.e. CID and HCD-based fragmentation) of purified spectral species. The complexity of both the experimental and computational steps involved in achieving DMA necessitates careful consideration of how to manage the large volumes of data generated, as well as novel computational tools to facilitate analysis. Here we introduce a set of novel tools and a workflow for the annotation of MSn data as well as a suite of web-based applications, collectively named MOGI (Metabolomics Organisation with Galaxy and ISA), to support DMA efforts. The latter provides a database and web-based platform for both managing and organising large-scale DMA data analyses, and associated results, using Galaxy-based workflows, while integrating these analyses within the ISA (Investigation, Study and Assay) framework.
We demonstrate the successful application of the DMA workflow in advancing understanding of the metabolome of Daphnia magna - a keystone species of freshwater ecosystems and NIH model organism for human health.
Silke Grabherr: Forensic Imaging and the Role of Magnetic Resonance Spectrometry in Forensic Medicine
University Center of Legal Medicine Lausanne-Geneva
Forensic Imaging has become an important field in forensic medicine. In many countries, especially in Europe and in Asia it is regularly used as adjunct to conventional forensic and medico-legal investigations in order to document and examine a human body after death or to perform a non-invasive documentation of lesions on living persons (victims or perpetrators).
Different technological methods are today used to perform forensic imaging. Most of them are issues from clinical radiology, but also other techniques, such as 3D-surface scanning are part of the applied panel. Among the radiological techniques, Multi-Detector Computed Tomography (MDCT) is the most often used. It is therefore well known as Post-mortem Computed Tomography (PMCT). In order to render it more informative, it can be combined with minimally invasive approaches such as post-mortem angiography (PMCTA) or post-mortem imaging-guided sample collection.
Magnetic Resonance Imaging (MRI) also plays in interesting role in Forensic Imaging, although its application is mostly limited to research purposes. Currently, it is only rarely applied as routing investigation. Examples of its application are the investigation of the structures of the neck for living victims that suffered an attempted strangulation and post-mortem investigations of deceased new-borns and fetuses. In these cases, it represents an alternative to a conventional autopsy that is most of the time refused by the grieving family. Main research fields in Post-mortem MRI (PMRI) are the investigation of the brain in cases of asphyxia and of cardiac tissue in cases of sudden cardiac death.
Beside this, investigations have also been made by using Magnetic Resonance Spectrometry (MRS). The existing literature points towards two main interests: investigating the post-mortem interval (in order to estimate the time of death) and investigating the presence of foreign components within the body (research for drugs or other substances with an interest in forensic toxicology). By using proton magnetic resonance spectroscopy ((1) H MRS), it is therefore possible to detect substances such as cocaine within the examined matrices. This allows performing drug research within the body and objects of forensic interest (e.g. smuggling of dissolved drugs).On the other hand, the observation of concentration changes of metabolites, usually observed in vivo in brain tissue, and the appearance of decay products was tested in order to eventually estimate the post-mortem interval.
This presentation shall give an overview of the current role of forensic imaging worldwide and in Switzerland. A special focus is put on forensic applications of MRI and MRS.